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KMID : 0381219750070050301
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Journal of RIMSK
1975 Volume.7 No. 5 p.301 ~ p.332
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The Fixation of Central Nervous Tissue and the Analysis of Electron
Micrographs of the Neurophil and Synaptic Organization
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Abstract
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The morphological identification by electron microscope of neurons, neuroglia cells and their processes, and synaptic organization is greatly helpful to the neurobiologist who studies the relationship between the nuclei of the central nervous system.
Although many workers have studied normal brain tissues with electron microscope, the results, considering the results attained on the other, organs, are still far from satisfactory. Author believes that the results achieved so far must be complemented by ever developing special techniques. Although, it is possible with the electron microscope to observe most of the fine structures of which have individual charateristics, the sections are so thin that they are suitable for plane observation but not suited for the understanding of three-dimensional relationship.
Complete three-dimensional observation is, of course, possible by means of the reconstruction of serial sections. However, this method is possible only when the tissue under study is small, but, it is improper when the object of study is a large neuropil.
Accordingly, it is believed that three-dimensional study of brain tissues is possible by par-´ ailed use of light and electron microscopy.
The possibility determining the origin of afferent fibers is in reality very small when the study made only by the morphological method, but the indirect method is very popular in which damage is inflicted upon a certain region of the brain tissue, and the necrosis of afferent axon terminals is examined by the electron microscope (Walberg, 1966: Heimer and Wall. 1968, Bak et al. 1975: Choi, et al., 1975).
It is believed that the afore-mentioned various methods and techniques will serve as valuable guide for studying and understanding the central nervous system.
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